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blasticidin  (Thermo Fisher)


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    Structured Review

    Thermo Fisher blasticidin
    Blasticidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hepes+buffer/Blasticidin+S+hydrochloride%2C+98%2B%25%2C+in+1M+HEPES+buffer+soln/pm42259351-86-15-21
    Average 97 stars, based on 1 article reviews
    blasticidin - by Bioz Stars, 2026-10
    97/100 stars

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    Cell Culture:

    Article Title: Conserved T cell receptor usage underpins recognition of CD1c presenting a mycobacterial lipid
    Article Snippet: .. Magnetically-enriched cells were cultured overnight in complete media (RPMI-1640 supplemented with 10% fetal bovine serum (FBS) (JRH Biosciences), penicillin (100 U/ml), streptomycin (100 μg/ml), GlutaMAX (2 mM), sodium pyruvate (1 mM), nonessential amino acids (0.1 mM), Hepes buffer (15 mM) (pH 7.2 to 7.5) (Invitrogen, Life Technologies), and 2 mercaptoethanol (50 μM, Sigma-Aldrich)) supplemented with 50 U/mL recombinant human IL-2 (Peprotech). .. Cells were then harvested, stained as above and FACS-Sorted using an ARIA III (Becton Dickinson).

    Recombinant:

    Article Title: Conserved T cell receptor usage underpins recognition of CD1c presenting a mycobacterial lipid
    Article Snippet: .. Magnetically-enriched cells were cultured overnight in complete media (RPMI-1640 supplemented with 10% fetal bovine serum (FBS) (JRH Biosciences), penicillin (100 U/ml), streptomycin (100 μg/ml), GlutaMAX (2 mM), sodium pyruvate (1 mM), nonessential amino acids (0.1 mM), Hepes buffer (15 mM) (pH 7.2 to 7.5) (Invitrogen, Life Technologies), and 2 mercaptoethanol (50 μM, Sigma-Aldrich)) supplemented with 50 U/mL recombinant human IL-2 (Peprotech). .. Cells were then harvested, stained as above and FACS-Sorted using an ARIA III (Becton Dickinson).

    Concentration Assay:

    Article Title: Biomimetic Nanogels Programmed for Irreversible-Electroporation-Primed Tumor Microenvironments to Elicit Durable Antitumor Immunity
    Article Snippet: .. The dried lipid film was hydrated at 37 °C for 1 h with 20 mM HEPES buffer (Thermo Fisher Scientific, 15630-056) at a total lipid concentration of 2 mg/ml. .. Finally, the liposome suspension was extruded 11 times through a 200-nm polycarbonate membrane (Avanti Polar Lipids) to obtain uniformly sized vesicles.

    Coagulation:

    Article Title: Poly(sulfobetaine- co -oligoethylene Glycol Methyl Ether Methacrylate) Copolymers with Improved Anti-Fouling and Anti-Coagulant Properties.
    Article Snippet: Zwitterionic polymers such as poly(sulfobetaines) and polyether polymers such as poly(ethylene glycol) (PEG) have both been reported as effective antifouling materials for various biomedical applications based on their high-waterbinding capacities through hydrogen bonding (PEG) or ion−dipole interactions (zwitterionic polymers).. Herein, to assess whether synergistic antifouling properties can be achieved when poly(sulfobetaine) and PEG moieties are combined into a single polymer, linear copolymers were fabricated by the chain transfer radical copolymerization of [2-(methacryloyloxy) ethyl] dimethyl-(3-sulfopropyl) ammonium hydroxide (DMAPS) and oligo(ethylene glycol) methyl ether methacrylate (OEGMA) together with a functional comonomer that introduced an aldehyde or hydrazide functional group to enable in situ gelation via hydrazone cross-linking.. In general, hydrogels prepared with more OEGMA showed longer gelation times, increased protein uptake (because of their higher degree of swelling), and faster plasma clotting times.

    Clinical Proteomics:

    Article Title: Poly(sulfobetaine- co -oligoethylene Glycol Methyl Ether Methacrylate) Copolymers with Improved Anti-Fouling and Anti-Coagulant Properties.
    Article Snippet: Zwitterionic polymers such as poly(sulfobetaines) and polyether polymers such as poly(ethylene glycol) (PEG) have both been reported as effective antifouling materials for various biomedical applications based on their high-waterbinding capacities through hydrogen bonding (PEG) or ion−dipole interactions (zwitterionic polymers).. Herein, to assess whether synergistic antifouling properties can be achieved when poly(sulfobetaine) and PEG moieties are combined into a single polymer, linear copolymers were fabricated by the chain transfer radical copolymerization of [2-(methacryloyloxy) ethyl] dimethyl-(3-sulfopropyl) ammonium hydroxide (DMAPS) and oligo(ethylene glycol) methyl ether methacrylate (OEGMA) together with a functional comonomer that introduced an aldehyde or hydrazide functional group to enable in situ gelation via hydrazone cross-linking.. In general, hydrogels prepared with more OEGMA showed longer gelation times, increased protein uptake (because of their higher degree of swelling), and faster plasma clotting times.

    Purification:

    Article Title: Inherent tissue homeostasis of the juvenile metaphysis provides a foundation for osteosarcoma development.
    Article Snippet: All enzymes were purchased from New England Biolabs. .. Following overnight digestion at 37 °C, the DNA was purified by ethanol precipitation, and the resulting pellets were resuspended in 100 μL of 25 mM HEPES buffer (Gibco). .. V6.5 ESCs were dissociated using 0.25% trypsin (Nacalai Tesque) and resuspended in 500 μL of high- glucose DMEM (Nacalai Tesque) supplemented with 25 mM HEPES 55.

    Ethanol Precipitation:

    Article Title: Inherent tissue homeostasis of the juvenile metaphysis provides a foundation for osteosarcoma development.
    Article Snippet: All enzymes were purchased from New England Biolabs. .. Following overnight digestion at 37 °C, the DNA was purified by ethanol precipitation, and the resulting pellets were resuspended in 100 μL of 25 mM HEPES buffer (Gibco). .. V6.5 ESCs were dissociated using 0.25% trypsin (Nacalai Tesque) and resuspended in 500 μL of high- glucose DMEM (Nacalai Tesque) supplemented with 25 mM HEPES 55.



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